{"id":20179,"date":"2022-05-31T07:55:46","date_gmt":"2022-05-31T05:55:46","guid":{"rendered":"https:\/\/www.izb-online.de\/neue-methode-revolutioniert-krebsdiagnose\/"},"modified":"2022-05-31T09:11:11","modified_gmt":"2022-05-31T07:11:11","slug":"neue-methode-revolutioniert-krebsdiagnose","status":"publish","type":"post","link":"https:\/\/www.izb-online.de\/en\/izb-biotech-news\/new-method-revolutionizes-cancer-diagnosis\/","title":{"rendered":"New method revolutionizes cancer diagnosis"},"content":{"rendered":"

[vc_row el_class=”gco-magazine-container”][vc_column el_class=”bildspalte”][vc_row_inner][vc_column_inner offset=”vc_col-lg-12 vc_col-md-12″][vc_column_text el_class=”gco-magazine–title”]<\/p>\n

New method revolutionizes cancer diagnosis<\/h1>\n

[\/vc_column_text][vc_column_text el_class=”gco-magazine-lead”]The groundbreaking “Deep Visual Proteomics” technology provides cell-specific, protein-based information and helps to analyze cancer diseases[\/vc_column_text][vc_column_text el_class=”gco-magazine–post-date”]May 2022[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_single_image image=”20174″ img_size=”full”][vc_column_text el_class=”gco-magazine-copyright”]\u00a9 MPI of Biochemistry[\/vc_column_text][vc_column_text el_class=”gco-magazine-picture-discription”]Deep Visual Proteomics concept and workflow Clockwise.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner el_class=”gco-magazine–quote” css=”.vc_custom_1611310713611{margin-top: 33px !important;}”][vc_column_inner][vc_column_text]<\/p>\n

\u201cWhen something goes wrong inside our cells and we become sick, you can be sure that proteins are involved in a wide range of different ways. Because of this, mapping the protein landscape can help us determine why a tumor could develop in a particular patient, what vulnerabilities that tumor has and also what treatment strategy might prove the most beneficial.\u201d<\/h6>\n

Matthias Mann
\nMax Planck Institute of Biochemistry, near Munich and at Novo Nordisk Foundation Center for Protein research<\/p>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner el_class=”gco-magazine-container–copy” css=”.vc_custom_1591795890737{margin-top: 21px !important;}”][vc_column_inner][vc_column_text]How does cancer arise? How does cellular composition influence tumor malignancy? These questions are profound and challenging to answer, but are crucial to understand the disease and find the right cure. Now, a German-Danish team led by Professor Matthias Mann has developed a ground-breaking technology called \u2018Deep Visual Proteomics\u2019. This method provides researchers and clinicians with a protein read-out to understand cancer at single cell-type resolution. The technology was published in the journal\u00a0Nature Biotechnology<\/em>\u00a0and demonstrates its potential in a first application to cancer cells.<\/strong><\/p>\n

Proteins are among the most important players in a variety of diseases. Aptly referred to as the ‘molecular workhorses of the cell\u2019, their proper function often determines the fitness of a cell and that of an individual by extension.<\/p>\n

Matthias Mann explains: \u201cWhen something goes wrong inside our cells and we become sick, you can be sure that proteins are involved in a wide range of different ways. Because of this, mapping the protein landscape can help us determine why a tumor could develop in a particular patient, what vulnerabilities that tumor has and also what treatment strategy might prove the most beneficial.\u201d<\/p>\n

Intrigued by these questions, a team of multi-disciplinary scientists from the research groups headed by Matthias Mann at the\u00a0Max Planck Institute (MPI) of Biochemistry, near Munich and at the\u00a0Novo Nordisk Foundation Center for Protein research (CPR)\u00a0at University of Copenhagen have invented an innovative method called \u2018Deep Visual Proteomics\u2019. The study combines visual features of a tumor with a deep profiling technology to visualize the protein signatures in aberrant cells that are adjacent to the surrounding healthy cells. This would give the researchers an unprecedented insight into the disease and guide oncologists to develop clever strategies for diagnosis and treatment.<\/p>\n

Deep Visual Proteomics unites four technologies<\/strong>
\nDeep Visual Proteomics integrates advances from four different technologies for the first time into a single workflow: First, advanced microscopy generates high-resolution tissue maps. Second, machine learning and artificial intelligence (AI) algorithms are used to accurately classify cells in shape, size, or protein localization before single cells are collected by highly-accurate laser capture microdissection. Then, after sorting normal or different types of diseased cells, the thousands of proteins present in the cell populations are detected all at once by using ultra-high sensitive mass spectrometry (MS) instruments from this minute amount of sample. Lastly, sophisticated bioinformatic analyses generate maps of proteins that provide spatial resolution of protein signatures across highly complex diseases such as cancer. Such protein landscapes are valuable tools for clinicians in understanding of mechanisms in health and disease in a greater detail.<\/p>\n

\u201cOur new concept, \u2018Deep Visual Proteomics\u2019, could become a game changer for molecular pathology in the hospitals. With this method, we take a tissue sample with tumor cells, and can identify and determine thousands of proteins in a minute of time and effort. These proteomic signatures reveal the mechanisms that drive tumor development and directly expose new therapeutic targets from a single tissue slice of a cancer patient biopsy. It exposes a cosmos of molecules inside these cancer cells,\u201d says Andreas Mund, Associate Professor at CPR and part of Professor Matthias Mann\u2019s team, that spearheaded this development.<\/p>\n

Relevance for clinical pathology<\/strong>
\nIn the new study, the researchers applied \u2018Deep Visual Proteomics\u2019 to cells from patients with acinic cell carcinoma and with melanoma. Lise Mette Rahbek Gjerdrum, consultant and clinical research associate professor at Department of Pathology, Zealand University Hospital and Department of Clinical Medicine, University of Copenhagen, describes: \u201cThis unique method combines tissue architecture with the expression of thousands of proteins specific for selected cells. It enables researchers to investigate interactions between cancer cells and their surrounding cells with major implications for future clinical cancer treatment. Recently, we diagnosed a highly complex clinical case using \u2018Deep Visual Proteomics\u2019.\u2019\u2019<\/p>\n

Dr. Fabian Coscia, co-first author of the study and \u2013 since June 2021 \u2013 head of the research group the “Spatial Proteomics” at the\u00a0Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC)\u00a0in Berlin, says: “But of course, the technique can be used to characterize all other tumor types in similar details.” His goal, he says, is to tap into the archived patient biobanks and find new targets for individualized, i.e., patient-tailored cancer therapies – even for previously treatment-resistant tumors.<\/p>\n

And it is not only cancer that can be better understood using \u2018Deep Visual Proteomics\u2019. The method can also be applied to other diseases. “For example, you can analyze the proteins in nerve cells to find out exactly what happens in the cells in neurodegenerative diseases such as Alzheimer’s or Parkinson’s.” says Fabian Coscia further.<\/p>\n

\u2018\u2019By merging the power of microscopy, AI and ultra-sensitive MS-based proteomics, we have developed a method that is very powerful to understand the molecular wiring of healthy versus disease cells. This could help doctors identify targets for future drugs and diagnostics.\u2019\u2019 concludes Matthias Mann.[\/vc_column_text][vc_column_text css=”.vc_custom_1583761043671{background-color: #1e73be !important;}” el_class=”redaktionell”][\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\n\n

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