{"id":16845,"date":"2021-04-20T18:07:57","date_gmt":"2021-04-20T16:07:57","guid":{"rendered":"https:\/\/www.izb-online.de\/mpi-fuer-biochemie-entwickelt-neue-methode-zur-analyse-von-dna-doppelstrangbruechen\/"},"modified":"2021-04-20T18:15:42","modified_gmt":"2021-04-20T16:15:42","slug":"mpi-fuer-biochemie-entwickelt-neue-methode-zur-analyse-von-dna-doppelstrangbruechen","status":"publish","type":"post","link":"https:\/\/www.izb-online.de\/en\/mpi-for-biochemistry-develops-new-method-to-analyze-dna-double-strand-breaks\/","title":{"rendered":"MPI for Biochemistry develops new method to analyze DNA double-strand breaks"},"content":{"rendered":"

[vc_row el_class=”gco-magazine-container”][vc_column el_class=”bildspalte”][vc_row_inner][vc_column_inner offset=”vc_col-lg-12 vc_col-md-12″][vc_column_text el_class=”gco-magazine–title”]<\/p>\n

MPI for Biochemistry develops new method to analyze DNA double-strand breaks<\/h1>\n

[\/vc_column_text][vc_column_text el_class=”gco-magazine-lead”]New technique based on next generation sequencing determines whether a protein binds to single-stranded or double-stranded DNA.[\/vc_column_text][vc_column_text el_class=”gco-magazine–post-date”]April 2021[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner el_class=”two-columns”][vc_column_inner el_class=”bild-zwei-spalten-links”][vc_single_image image=”16838″ img_size=”full”][vc_column_text el_class=”gco-magazine-copyright”]\u00a9 rost9, AdobeStock\/ modifiziert M. Peritore, MPI f\u00fcr Biochemie[\/vc_column_text][vc_column_text el_class=”gco-magazine-picture-discription”]Molekulare Prozesse an einem DNA Doppelstrangbruch.[\/vc_column_text][vc_single_image image=”16836″ img_size=”full”][vc_column_text el_class=”gco-magazine-copyright”]\u00a9 Susanne Vondenbusch-Teetz, MPI f\u00fcr Biochemie[\/vc_column_text][vc_column_text el_class=”gco-magazine-picture-discription”]Dr. Boris Pfander,<\/strong> Leiter der Forschungsgruppe \u201eDNA-Replikation und Genomintegrit\u00e4t\u201c am Max-Planck-Institut f\u00fcr Biochemie.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner el_class=”gco-magazine–quote” css=”.vc_custom_1611310713611{margin-top: 33px !important;}”][vc_column_inner][vc_column_text]<\/p>\n

\u201cWith the help of this ChIP-seq method, we were able to determine whether a protein binds to the single stranded or double stranded DNA domain,\u201d explained Dr. Boris Pfander, head of the research group “DNA Replication and Genome Integrity” at the Max Planck Institute of Biochemistry.\u201d<\/h6>\n

Dr. Boris Pfander
\nHead of the research group “DNA Replication and Genome Integrity” at the Max Planck Institute of Biochemistry<\/p>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner el_class=”gco-magazine-container–copy” css=”.vc_custom_1591795890737{margin-top: 21px !important;}”][vc_column_inner][vc_column_text]DNA in our cells is constantly exposed to damaging agents, such as UV light or byproducts of cellular metabolism like reactive oxygen species. The most severe form of DNA damage are DNA double stranded breaks. In order to keep the genetic information intact, cells need to repair these double stranded breaks. The most faithful repair process is homologous recombination, which involves DNA end resection, the degradation of one DNA strand at each side of the break. Two different domains are therefore created around the double strand breaks: one containing single-stranded DNA and one containing double-stranded. Researchers from the team of Boris Pfander, head of the research group “DNA Replication and Genome Integrity” at the Max Planck Institute of Biochemistry have now developed a ChIP-seq-technique, based on chromatin-immunoprecipitation followed by next-generation sequencing. With the help of this ChIP-seq method, the researchers were able to determine whether a protein binds to the single stranded or double stranded DNA domain. Therefore, they could shed light on the fate of nucleosomes, the fundamental unit of chromatin, during DNA end resection. Martina Peritore, first author of the study, explains, “Previous\u00a0in vitro<\/em>\u00a0data have shown that nucleosomes can form not only on double-stranded DNA but also on single-stranded DNA. In contrast, we observed that in yeast cells nucleosomes are removed from DNA during resection. That is, DNA resection and nucleosome removal are coupled.” These results suggest that the epigenetic information associated to nucleosomes is lost once cells enable resection. Peritore continued, \u201cOur work therefore raises the question of how such information is restored after a DSB has been repaired\u201d. The study was published in the scientific journal\u00a0Molecular Cell.<\/em>[\/vc_column_text][vc_column_text css=”.vc_custom_1583761043671{background-color: #1e73be !important;}” el_class=”redaktionell”][\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\n\n

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